PlantScreen高通量植物表型成像分析平臺（傳送帶版）（二）

10.根系成像分析

·RhizoTron根窗技術(shù)，全自動成像分析，標(biāo)配根窗44x29.5x5.8cm（高x寬x厚度）

·不僅可對根系成像分析，還可對地上苗（shoot）進行成像分析，苗高大50cm

·新一代CMOS傳感器，分辨率12.3MP

·均一LED光源

·3層定位（頂部、中部、底部）根系澆灌系統(tǒng)（選配），3個水箱獨立運行

·測量參數(shù)包括：根深（或高度）、根冠寬度、高度與寬度比值、根冠面積、根冠緊實度、根系總長、軸對稱性、根尖數(shù)、根節(jié)數(shù)等

11.自動澆灌與稱重單元

·測量參數(shù)：實際重量、澆水體積、終重量、每個培養(yǎng)盆的相對重量

·操作指令：每個培養(yǎng)盆澆相同量的水（克數(shù)或者實際重量的百分比）；保持相對重量；自定義每個培養(yǎng)盆的澆灌量模擬不同干旱或者內(nèi)澇脅迫；稱重前自動零校準(zhǔn)，還可通過已知重量（如砝碼）物品自動進行再校準(zhǔn)

·每個培養(yǎng)盆的澆水量、日期、時間可分別程序控制記錄以創(chuàng)建不同干旱脅迫梯度等，并且與整個系統(tǒng)的表型大數(shù)據(jù)無縫結(jié)合分析

·稱重精度：大型植物±2g，小型植物±0.2g

·澆灌單元：流速3L/min，澆灌口高度可自動上下前后調(diào)整，保證澆灌位置

12.自動化植物傳送系統(tǒng)

·傳送植物大?。焊鶕?jù)客戶需求，可達200cm

·傳送帶容納量：50盆植物（1000株小型植物），可擴展100盆、200盆、400盆等更大容量 ；表型分析通量依不同的protocol而定，100分鐘可以完成整個系統(tǒng)載荷植物樣品的表型分析，可隨機傳送至成像室進行成像分析、隨機澆灌

·培養(yǎng)盆：防UV聚丙烯材料，標(biāo)準(zhǔn)5L（口徑24cm）培養(yǎng)盆，可通過適配器應(yīng)用3L培養(yǎng)盆，可360度旋轉(zhuǎn)

·具備手動載樣環(huán)（manual loading loop）以便在系統(tǒng)待機模式下手動載樣分析實驗、小組實驗分析等

·具備激光植物高度測量監(jiān)測系統(tǒng)和*

·環(huán)形傳送通道：具變速箱的三相異步馬達，功率200-1000W，大負(fù)載500kg，速度150mm/s，傳送帶材料為防UV高耐用PVC

·移動控制系統(tǒng)：*處理單元CJ2M-CPU33；數(shù)字輸入/輸出大2560點；輸入/輸出單元大40；溫度傳感器Pt1000，Pt100，PTC；PLC通訊百兆以太網(wǎng)；OMRON MECHATROLINK-II 大16軸精確定位

·RFID標(biāo)簽和QR植物辨識系統(tǒng)，自動讀取每個樣品托盤上的二維編碼；辨識距離2-20cm；通訊RS485；可讀取1維、2維和QR碼；配備LED光源便于弱光下辨識

·環(huán)境監(jiān)測傳感器：溫濕度傳感器、PAR光合有效輻射傳感器

·由主控制系統(tǒng)分別自動調(diào)控每一個樣品托盤的測量時間、測量順序、測量參數(shù)、澆灌時間和澆灌量，從測量單元到培養(yǎng)室的樣品運轉(zhuǎn)整個過程可實現(xiàn)*自動控制，在無人值守情況下根據(jù)預(yù)設(shè)程序自行完成全部實驗測量工作。

13.主控制表型大數(shù)據(jù)平臺

·組成：控制調(diào)度服務(wù)器、客戶端應(yīng)用服務(wù)器、數(shù)據(jù)服務(wù)器、可編程序邏輯控制器及專業(yè)分析軟件等，數(shù)據(jù)容量12TB

·自動控制與分析功能：具備用戶定義、可編輯自動測量程序（protocols），根據(jù)用戶設(shè)定程序自動完成全部實驗。數(shù)據(jù)結(jié)果自動存儲并分析，分析的數(shù)據(jù)結(jié)果可自動以動態(tài)曲線的形式顯示。

·MySQL數(shù)據(jù)庫管理系統(tǒng)，可以處理擁有上千萬條記錄的大型數(shù)據(jù)庫，支持多種存儲引擎，相關(guān)數(shù)據(jù)自動存儲于數(shù)據(jù)庫中的不同表中

·植物編碼注冊功能：包括植物識別碼、所在托盤的識別碼等存儲在數(shù)據(jù)庫中，測量時自動提取自動讀取條形碼或RFID標(biāo)簽

·觸摸屏操作界面，在線顯示植物托盤數(shù)量、光線強度、分析測量狀態(tài)及結(jié)果等，輕松通過軟件*控制所有的機械部件和成像工作站

·可用默認(rèn)程序進行所有測量，也可通過開發(fā)工具創(chuàng)建自定義的工作過程，或者手動操作LED光源開啟或關(guān)閉、RGB成像、葉綠素?zé)晒獬上?、高光譜成像、紅外熱成像、3D激光掃描、稱重及澆灌等

·葉片跟蹤監(jiān)測功能（leaf tracking）模塊，可以持續(xù)跟蹤監(jiān)測葉片的生長、變化等等

·3D投射技術(shù)，可以通過高分辨率RGB鏡頭 或激光掃描構(gòu)建3D模型，通過投射技術(shù)，將與其它傳感器所得數(shù)據(jù)如葉綠素?zé)晒?、紅外熱成像溫度數(shù)據(jù)、近紅外數(shù)據(jù)、高光譜數(shù)據(jù)等投射在3D模型上一起進行對比分析等

·允許用戶通過互聯(lián)網(wǎng)遠(yuǎn)程訪問，進行數(shù)據(jù)處理、下載及更改實驗設(shè)計

·所測量的所有數(shù)據(jù)都是透明的、可以追溯的

·具備用戶權(quán)限分級功能，防止其他人員誤操作影響實驗

·廠家遠(yuǎn)程故障診斷，軟件*升級

執(zhí)行標(biāo)準(zhǔn)：

·CE認(rèn)證標(biāo)準(zhǔn)

·CSN EN 60529 防護等級標(biāo)準(zhǔn)

·CSN 33 01 65 導(dǎo)體側(cè)識別標(biāo)準(zhǔn)

·CSN 33 2000-3 基礎(chǔ)特性標(biāo)準(zhǔn)

·CSN 33 2000-4-41ed.2 電擊保護標(biāo)準(zhǔn)

·CSN 33 2000-4-43 電源過載保護標(biāo)準(zhǔn)

·CSN 33 2000-5-51ed.2 通用規(guī)則標(biāo)準(zhǔn)

·CSN 33 2000-5-523 容許電流標(biāo)準(zhǔn)

·CSN 33 2000-5-54ed.2 接地與保護導(dǎo)體標(biāo)準(zhǔn)

·CSN EN 55011 工業(yè)、科學(xué)與醫(yī)學(xué)設(shè)備測量電磁干擾的范圍與方法

·2006/42/EG 機械指令標(biāo)準(zhǔn)

·73/23/EEG 低電壓指令標(biāo)準(zhǔn)

·2004/108/EG 電磁相容性指令標(biāo)準(zhǔn)

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附：其它表型分析平臺：

1、FKM多光譜熒光動態(tài)顯微成像系統(tǒng)

右圖引自《Nature Plants》2016, Photonic multilayer structure of Begonia chloroplasts enhances photosynthetic efficiency by Heather M. Whitney等

2、PlantScreen-R移動式表型分析平臺（下左圖）：用于大田植物葉綠素?zé)晒獬上穹治?、RGB成像分析、紅外熱成像分析、3D激光掃描測量分析等

3、PlantScreen臺式及移動式植物表型分析平臺（參見上右圖）

1)3D RGB彩色成像分析

2)FluorCam葉綠素?zé)晒獬上穹治?/span>

3)FluorCam多光譜熒光成像分析

4)高光譜成像分析

5)紅外熱成像分析

6)PAR吸收／NDVI成像分析

7)近紅外3D成像分析

4、PlantScreen樣帶式表型分析平臺

5、PlantScreen 植物表型三維自動掃描成像分析平臺