37360 - 疼痛甩尾
疼痛甩尾主要是用于測量大、小鼠尾巴部受紅外熱刺激的痛覺閾值。實(shí)驗(yàn)時(shí)，當(dāng)動(dòng)物感覺疼痛，尾巴會(huì)輕敲臺(tái)面，內(nèi)置傳感器會(huì)立刻檢測到，停止計(jì)時(shí)和關(guān)閉光源，即儀器自動(dòng)記錄反應(yīng)時(shí)間和光源強(qiáng)度。數(shù)據(jù)可通過U盤、USB數(shù)據(jù)線等導(dǎo)出。
該儀器中有一可調(diào)強(qiáng)度的紅外光源，其紅外光主要是通過一拋物面反射鏡聚焦在實(shí)驗(yàn)動(dòng)物的尾巴上。實(shí)驗(yàn)時(shí)，操作人員將實(shí)驗(yàn)動(dòng)物置于儀器上，把動(dòng)物的尾巴放在紅外光源處，接受刺激。
該儀器也可以在小鼠上進(jìn)行實(shí)驗(yàn)，有小鼠設(shè)備配件可供選擇。
延遲時(shí)間和強(qiáng)度能夠通過USB或者串行端口導(dǎo)出。USB和軟件都包含在內(nèi)。
| 特征 | 優(yōu)點(diǎn) |
| 儀器自動(dòng)記錄實(shí)驗(yàn)數(shù)據(jù) | 精度高，避免人為因素引起的誤差 |
| 包含U盤和軟件 | 可獨(dú)立工作，也可連接電腦使用 |
| 儀器工作臺(tái)表面無突出和遮擋物件 | 操作方便，實(shí)驗(yàn)重復(fù)性好 |
規(guī)格：
| 命令： | 軟鍵和腳踏板 | | 連接電腦： | DELTA 9-pin連接頭，USB連線 |
| 數(shù)據(jù)讀?。?/p> | 液晶顯示 | | 電源： | universal mains 85-264 VAC, 50-60Hz |
| 打?。?/p> | 微型熱敏打印機(jī)（需另外購買） | | 工作溫度： | 15° - 30° C |
| 開始： | 紅外開關(guān) | | 聲級(jí)： | < 70 dB |
| 紅外強(qiáng)度： | 10-99級(jí)間可調(diào) | | 紅外光源燈泡； | Halogen "Bellaphot", Mod. 64607 OSRAM, 8V-50W |
| 反應(yīng)時(shí)間： | 液晶屏顯示，分辨率為0.1s | | 校準(zhǔn)： | 紅外熱輻射計(jì)（需另外購買） |
| 截止時(shí)間： | 預(yù)置，15 - 60 s間 | | | |
附件：
| 37360-325: | 小鼠束縛器，25 mm I.D. |
| 37360-330: E-HR 002: | 小鼠束縛器，30 mm I.D. 替換燈泡 |
| 37300 | 紅外熱輻射計(jì) |
| 57145 | 微型打印機(jī) |
參考文獻(xiàn)
方法
- F.E. D’Amour & D.L. Smith: "A Method for Determining Loss of Pain Sensation." J. Pharmacol. Exp. Therap. 72: 74-79, 1941.
涉及UB的甩尾實(shí)驗(yàn)
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- P.J. McLaughlin et alia: “Behavioral Effects of the Novel Potent Cannabinoid CB1 Agonist AM 4054”Pharmacology Biochemistry and Behavior 109: 16-22, 2013
- T.A. Kosten et alia: “A Morphine Conjugate Vaccine Attenuates the Behavioral Effects of Morphine in Rats” Progr. in Neuro-Psychopharmacol. and Biol. Psychiatry 45: 223–229, 2013
- T.C. Chen et alia: “Spontaneous inflammatory Pain Model From a Mouse Line With N-ethyl-N-nitrosourea Mutagenesis” J. Biomed. Science 19 (55): 2–15, 2012
- J. Walsh et alia: “Disruption of Thermal Nociceptive Behaviour in Mice Mutant for the Schizophrenia-Associated Genes NRG1, COMT and DISC1” Brain Res. 1348: 114-119, 2012
- K. Guillemyn et alia: “In vivo Antinociception of Potent mu Opioid Agonist Tetrapeptide Analogues and Comparison with a Compact Opioid Agonist-neurokin 1 Receptor Antagonist Chimera” Molecular Brain5 (4): 2-11, 2012
- A.J. Morrison et alia: “Design, Synthesis, and Structure–Activity Relationships of indole-3-heterocycles as Agonists of the CB1 Receptor” Bioorganic & Medicinal Chemistry Letters 21: 506-509, 2011
- M. Spetea et alia: “In vitro and in vivo Pharmacological Profile of the 5-benzyl Analogue of 14-methoxymetopon, a Novel μ Opioid Analgesic with Reduced Propensity to Alter Motor Function” Eur. J. Pharmac. Sciences 41: 125-135, 2010
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- M.A. Philips et alia: “Myg1-Deficient Mice Display Alterations in Stress-Induced Responses and Reduction of Sex-Dependent Behavioural Differences” Behav. Brain Res. 207: 182-195, 2010
- C. Dawson et alia: “ Dexmedetomidine Enhances Analgesic Action of Nitrous Oxide” Anesthesiology 100 (4): 894−904, 2004
- P. Tolu et alia: “ Effects of Long-Term Acetyl-L-carnitine Administation in Rats: I. Increased Dopamine Output in Mesocorticolimbic Areas and Protection Toward Acute Stress Exposure” Neuropsychopharmacol. 27 (3): 410-420, 2002
- R. Nadeson et alia: “ Potentiation by Ketamine of * Antinociception. I. An Experimental Study in Rats Showing that Ketamine Administered by Non-Spinal Routes Targets Spinal Cord Antinociceptive Systems” Br. J. Anaesthesia 88 (5): 685−691, 2002
- L. Jasmin et alia: “ The NK1 Receptor mediates Both the Hyperalgesia and the Resistance to Morphine in Mice Lacking Noradrenaline” PNAS 99 (2): 1029−1034, 2002
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- M. Xu et alia: “ Effects of Radolmidine, a Novel α2- Adrenergic Agonist Compared with Dexmedetomidine in Different Pain Models in the Rat” Anesthesiology 93 (2): 473−481, 2000
- A. Köster et alia: “Targeted Disruption of the Orphanin Fq/Nociceptin Gene Increases Stress Susceptibility and Impairs Stress Adaptation In Mice” Neurobiology 96 (18): 10444-10449, 1999
- I. Sora et alia: “Opiate Receptor Knockout Mice Define µ Receptor Roles in Endogenous Nociceptive Responses and Morphine-Induced Analgesia” Neurobiology 94: 1544-1549, 1997
- C.T. Dourish et alia: "The Selective CCK-B Receptor Antagonist L-365,260 Enhances Morphine Analgesia and Prevents Morphine Tolerance in the Rat" Europ. J. Pharmacol. 176: 35-44, 1990
- P.W. Nance & J. Sawinok: "Substance P-Induced Long-Term Blockade of Spinal Adrenergic Analgesia: Reversal by Morphine and Naloxone" J. Pharmacol. Exp. Therap. Vol. 240, No. 3: 972-977, 1987
